Journal: Molecular Medicine Reports

Article Title: Acute carbon monoxide poisoning induces renal inflammation and apoptosis via CCL4 upregulation and activation of the PI3K/Akt pathway in mice

doi: 10.3892/mmr.2025.13709

Figure Lengend Snippet: ACOP induces apoptosis in kidney tissues and triggers systemic inflammatory responses in mice. (A) Western blot analysis was performed to determine the expression levels of CCL4, p-Akt/Akt, Bax and Bcl-2 in renal tissues from the control and model groups. Statistical evaluation was also performed. (B) Apoptosis in kidney tissues from mice in the control and model groups was assessed by TUNEL staining. (C) Serum levels of the inflammatory cytokines TNF-α, IL-6 and IL-1β in mice were measured using ELISA. Data are expressed as the mean ± SD (n=3). **P<0.01 vs. control. CCL4, C-C motif chemokine ligand 4; ACOP, acute carbon monoxide poisoning; p-, phosphorylated.

Article Snippet: The serum levels of TNF-α, IL-6 and IL-1β were quantified according to the manufacturer's instructions using Mouse TNF-α ELISA Kit (Order NO. D721217, Sangon Biotech, Shanghai, China), Mouse IL-6 ELISA Kit (Order NO. D721022, Sangon Biotech, Shanghai), and Mouse IL-1β ELISA kit (Order NO. D721017, Sangon Biotech, Shanghai, China).

Techniques: Western Blot, Expressing, Control, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay